Total Antioxidants Status, Selenium Concentration, Catalase and Glutathione Peroxidase Activities in Rabbits Fed a High-Garlic Diet | Chapter 11 | Recent Advances in Biological Research Vol. 4
Aim:
Results from previous studies on the effects of intake of garlic on the body
antioxidant system appear inconclusive. This study measured glutathione
peroxidase (GPx) and catalase (CAT) activities, Total Antioxidant Status (TAS)
and selenium (Se) level in rabbits fed a high-garlic diet.
Methods: Ten rabbits randomly
assigned into two groups (group 1=control and group 2= test) of five rabbits
each were used for the experiment. The diet of group 2 was mixed with raw
garlic homogenate (1.75g/Kg body weight/day) for 4 weeks. Two sets of blood
samples were collected from each animal at the beginning of the experiment
(baseline) and at the end (4 weeks). Blood samples were analyzed for blood GPx
and serum CAT activities and TAS by spectrophotometric methods while Se was
determined using Atomic Absorption Spectrophotometric (AAS) method.
Results:
At baseline, means of the parameters measured did not differ significantly
between groups 1 and 2. At the 4th week, means of TAS (1741.20±381.53 µmol
Trolox equiv/L), Se (47.20±12.22 µg/dl), CAT activity (273.20±68.05 U/L) and
GPx activity (12392.00±3068.34 U/L) in the test group were significantly higher
than means of the corresponding control group (820.20±91.94 µmol Trolox
equiv/L, 20.80±1.92 µg/dl, 145.40±13.35 U/L, 8528.00±1757.59 U/L,
respectively). There were positive associations between CAT and GPx (r = 0.65,
p<0.01) activities, CAT and TAS (r = 0.77, p<0.01), GPx and TAS (r =
0.66, p<0.01) and Se and TAS (r = 0.70, p<0.01).
Conclusion:
Results from this present study suggest that significant increases observed in
the activities of antioxidant enzymes, Se level and TAS could possibly be
associated with consumption of high garlic diet by the rabbits. Overall, the
significant increase in the CAT and GPx activities as well as the levels of Se
and TAS seen in the test group at the end of the study suggests that
consumption of raw garlic extract could result to increases in the activities
of important antioxidant enzymes and improve the total antioxidant status of
the body of rabbits.
Author(s) Details
Japhet M. Olisekodiaka
Department of Chemical
Pathology, College of Health Sciences, Nnamdi Azikiwe University, Awka,
Nigeria.
Christopher Igbeneghu
Department of Biomedical
Sciences, College of Health Sciences, Ladoke Akintola University of Technology,
Ogbomoso, Nigeria.
Adekunle A. Adesiyan
Department of Biomedical
Sciences, College of Health Sciences, Ladoke Akintola University of Technology,
Ogbomoso, Nigeria.
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