Correlation of GSTP1 Polymorphism with Severity of Prostate Cancer in an Eastern Indian Population | Chapter 13 | Emerging Research in Medical Sciences Vol. 1
Background:
GSTP1 is one of the Glutathione-S-Transferases (GSTs) which suppress tumor
genesis by detoxifying toxic carcinogens and reactive oxygen species (ROS).
Prostate cancer is related to several mutations affecting the expression of
GSTP1. A single nucleotide polymorphism (SNP: Ile105Val) in the GSTP1 gene
results insignificant reduction in its anticancer activity. The current case
control study was conducted to ascertain the risk of association of
GSTP1polymorphism with risk of cancer prostate in an Eastern Indian population.
Materials and Methods: During a
study period of 2 years, DNA was isolated using the phenol chloroform
extraction method from the blood of 225 histopathologically diagnosed prostate
cancer patients and 120 matched controls. The GSTP1 polymorphism was assessed
by PCR amplification of the gene followed by restriction digestion with Alw261
(a restriction enzyme derived from Acinetobactro lwoffi RFL26).
Histopathological grading in the case group was performed using Gleason’s
scores and International Society of Urological Pathology (ISUP) grading.
Results: Comparison of the
distribution of different GSTP1 alleles between the case and control groups was
performed by chi square test and odds ratio analysis. A χ2 value of 18.56
suggested significantly higher number of G alleles in the case group. An odds
ratio of 2.25 with a confidence interval of 1.52 to 3.34 for 95% CI showed that
the G allele in GSTP1 gene were linked with greater risk of prostate cancer.
Post hoc ANOVA and logistic regression suggested that cases having G alleles
had more progressive form of diseases as evident from ISUP grades.
Conclusion: From our study we can
conclude that GSTP1 polymorphism is not only significantly associated with risk
of prostate cancer but also with its severity in our Eastern Indian population.
GSTP1 polymorphism should be considered as a prognostic indicator for prostate
cancer patients along with planning for more aggressive management of the
disease.
Author(s) Details
Dr. Suparna Roy
Department of Biochemistry,
Calcutta National Medical College, Kolkata-14, India.
Dr. Anindya Dasgupta
Department of Biochemistry,
Calcutta National Medical College, Kolkata-14, India.
Miss Subarnarekha Chatterji
Department of Biotechnology,
School of Life Sciences, Manipal University, Manipal, India.
Dilip Karmakar
Department of Urology,
Calcutta National Medical College, Kolkata-14, India.
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