Screening for Oral High-risk Human Papillomavirus (HPV) among a Pediatric Patient Population | Chapter 10 | Emerging Research in Medical Sciences Vol. 2
Introduction:
Human papillomavirus (HPV) is a known cancer-causing virus that has been
primarily linked to cervical cancers. Recent studies have shown that HPV may
also induce cancer in other tissues, including oral epithelia and mucosa. To
determine the presence of oral HPV infection among pediatric patients, salivary
samples were assessed to determine oral prevalence of high-risk HPV.
Methods:
Using existing pediatric saliva samples from pediatric clinic patients (aged 6
- 16 years), this retrospective study involved isolating DNA for PCR screening
for both high-risk strains of HPV (HPV16, HPV18). A total of n=98 patient
samples were available for analysis.
Results:
Demographic analysis of these samples revealed the majority were derived from
females (63.2%) and mostly from Hispanics (54.1%). DNA was successfully
isolated from 95.9% of samples (n=94/98) with an average concentration of 142.5
ng/uL. PCR screening demonstrated only a subset of patient samples harbored
high-risk strains, HPV16 (n=9) and HPV18 (n=6), which represents 15.9% of the
total sample population - and were concentrated among the older patients
(>13 years old).
Conclusions:
Although this retrospective study does not have a sufficient sample size to
provide more robust analysis of other risk factors (race, sex,
sociodemographic), these results to demonstrate that oral HPV infection may be
successfully detected among a pediatric population. As more studies demonstrate
oral HPV infection in children, these data are of significant value to other
dental, medical, oral and public health professionals who seek to further an
understanding of oral health and disease risk in pediatric populations.
Author(s) Details
Douglas Bowen
Department of Advanced
Education in Pediatric Dentistry, Las Vegas – School of Dental Medicine,
University of Nevada, 1001 Shadow Lane, Las Vegas, Nevada, 89106, USA.
Dr. Karl Kingsley, (PhD,
MPH)
Department of Biomedical
Sciences, Las Vegas – School of Dental Medicine, University of Nevada, 1001
Shadow Lane, Las Vegas, Nevada, 89106, USA.
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