Skip to main content

Antibacterial Activity of Ocimum gratissimum (scent leaf) on Some Pathogenic Gastrointestinal Bacteria | Chapter 11 | Theory and Applications of Microbiology and Biotechnology Vol. 2

The emergence of antibiotic resistance as well as the recent undesirable side effect of some of the commercially available antibiotics has led to the screening of plant extract in order to discover new drug that could serve as alternative therapy for the treatment of various infections and diseases. Fresh leaf of Ocimum gratissimum (scent leaf) sample was collected, air-dried at room temperature and blended to powder using electric blender. The extraction was done using reflux extraction method and methanol as solvent. The phytochemical analysis and the antibacterial activity of O. gratissimum were determined to ascertain the different phytochemicals present in the plant extract. The extract was also tested against some selected Gram negative intestinal pathogenic bacteria; Escherichia coli, Shigella and Salmonella species, by reconstituting the extract in dimethyl sulphoxide (DMSO) to obtain different concentration (0.2, 0.1, 0.05 and 0.025 g/ml) and agar well diffusion techniques were used to evaluate the antibacterial susceptibility of the leaf extract. The qualitative phytochemical analysis of the extract revealed the presence of alkaloid, anthraquinone, flavonoid, glycoside, phenol, saponin, steroid and tannins. The result of antibacterial analysis showed that the extract of O. gratissimum has antibacterial activity against E. coli. This could be as a result of the presence of various phytochemicals or the interaction of one or more of the identified metabolites against the test organisms. However, there was no zone of inhibition (antibacterial effect) recorded on Salmonella and Shigella spp. as they were resistant to the extract. The results obtained from this research, suggest that Escherichia coli was susceptible to the leaf extract and the plant could be used as potential source of natural product for the treatment of infection.

Author(s) Details

Agholor Kin
Department of Biological Science, Niger State Polytechnic, Zungeru, Niger State, Nigeria.

Lucy M. Yaki
Department of Microbiology, Kaduna State University, Kaduna, Kaduna State, Nigeria.

Idris Abubakar
Department of Biological Science, Niger State Polytechnic, Zungeru, Niger State, Nigeria.

Lucy F. Olusola
Department of Biological Science, Niger State Polytechnic, Zungeru, Niger State, Nigeria.

Rakiya Zubairu
Department of Biological Science, Niger State Polytechnic, Zungeru, Niger State, Nigeria

View Book: http://bp.bookpi.org/index.php/bpi/catalog/book/107
Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

Study on Processing Method Influences the Effect of Cassava (Manihot esculenta) Consumption on Blood Lipid Profile in Rats | Chapter 8 | Current Research and Development in Chemistry Vol.1

Cassava is consumed in many tropical countries and is fast becoming the major raw material of many diets, especially in the bakery, brewery and pharmaceutical industries. In a study to determine the effects of various formulations of Cassava feeds on blood lipid profile in rats. Forty adult Wistar rats weighing between 120 and 150 g were divided into 4 groups of 10 rats each. The rats were acclimatized for one week; fed with different formulations of Cassava feeds adlibitum, after which the rats were allotted into groups. Group 1 was fed on Garri; group 2 on Cassava flour; group 3 on Tapioca, and the control group (group 4) on normal chow. After 4 weeks, each animal was anaesthetized by exposure to chloroform vapour and blood samples collected by cardiac puncture. Group 1 diet (Garri) significantly increased HDL-cholesterol (HDL-C) concentration and significantly decreased LDL-cholesterol (LDL-C) concentration (p<0.05) compared with the control group. In Group 2 (Cassava flour), t...
Post a Comment
Read more

Ethnopharmacological Survey among Traditional Medicine Practitioners in Abidjan (Côte d'Ivoire) for the Management of Pathologies such as Malaria, ENT Diseases, Diarrhea, Typhoid Fever and Anemia | Chapter 05 | New Horizons in Medicine and Medical Research Vol. 8

 Traditional Medicine Practitioners (TMPs) of Côte d'Ivoire are familiar with the therapeutic herbs. Medicinal plants have been shown to be useful in the treatment of common diseases such as malaria (KROA, 2004) and diabetes (N'GUESSAN et al, 2013) in several studies and scientific study. In the Abidjan District, the current policy is to integrate traditional medicine and pharmacopoeia into the national health system in order to improve population health coverage, particularly in the management of commonly encountered diseases such as malaria, ENT diseases, diarrhoea, typhoid fever, and anaemia. This research aims to increase the use of traditional medicine and pharmacopoeia in the national health system. Over the course of three months, fifty (50) PMTs in the District of Abidjan participated in this study. Plant therapists accounted for 30 of the 50 PMTs polled at the end of the study, or 60%. Traditional medicine centres use 61 types of medicinal plants from 36 famili...
Post a Comment
Read more