Antibiotic Resistant Profiles of Food (Fresh Raw Milk) and Environmental (Abattoir Effluents) Isolates of Listeria monocytogenes from the Six Zones of Nigeria | Chapter 14 | Theory and Applications of Microbiology and Biotechnology Vol. 2
The prevalence of Listeria
monocytogenes in fresh raw milk and abattoir effluents in the six zones of
Nigeria was determined. Antibiotic resistant profile of the isolates was
examined using the Bauer- Kirby disc diffusion assay. A total of 626 food and environmental
samples were cultured on selective media out of which 54 (8.6%) were positive
for L. monocytogenes. Chloramphenicol was the most effective antibiotic against
the isolates with the least resistance (3.70%) while nalidixic acid proved to
be least effective with resistance of 90.74%. The multiple-antibiotic resistant
pattern of the isolates showed nalidixic acid/cloxacillin (35.2%), nalidixic
acid/colistin (31.5%) and cloxacillin/colistin/nalidixic acid (29.6%) to be
most prominent. The least value was observed in
chloramphenicol/nitrofurantin/cotrimoxazole with 5.6%. The modal values of the
minimum inhibitory concentrations (MICs) of the antibiotics to the isolates
range between 4.0 and >16.0 µg/ml. Chloramphenicol, nitrofurantin and
gentamycin recorded the highest MIC compared with other antibiotics. This study
has demonstrated that a wide and rapidly expanding range of undesirable and, in
some cases, multi-resistant determinants is currently present in L.
monocytogenes.
Author(s) Details
Dr. L. U. Enurah
National Veterinary Research
Institute, Vom, Plateau State, Nigeria.
Prof. O. O. Aboaba
Department of Microbiology,
Faculty of Science, University of Lagos, Lagos, Nigeria.
S. C. U. Nwachukwu
Department of Microbiology,
Faculty of Science, University of Lagos, Lagos, Nigeria.
C. I. Nwosuh
National Veterinary Research
Institute, Vom, Plateau State, Nigeria.
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