Cyclic Depsipeptide Producing Fluorescent Pseudomonads Exerts Antifungal Activity against Fungal Pathogens of Maize (Zea mays) | Chapter 05 | Theory and Applications of Microbiology and Biotechnology Vol. 2
Cyclic lipopeptides (CLPs) with
antibiotic, biosurfactant producing fluorescent pseudomonads were isolated from
sugar beet-maize intercropped in sandy loam soils at Maize Research Station,
Vagarai, TNAU. Approximately 20 fluorescent pseudomonads from sandy loam soils
were isolated by using two different growth media. The strains were
distinguished based on their growth, CFU/g, fluorescence, and pigment
production. Growth inhibition of maize pathogens by CLP producing fluorescent
pseudomonads strains were studied by dual culture experiments. The impact of
CLP producing flurorescent pseudomonads strain on the zoospores of Downy mildew
pathogen of maize was studied by direct microscopy and encysted zoospores were
observed. Invitro, biochemical experiments confirmed the presence of
Viscosinamide producing strain among the fluorescent pseudomonads isolates in
terms of utilization of C and N sources. The particular strain was tested for
its growth promoting activity by treating the maize seeds for their
germination, and seedling vigour performance. Fluorescent pseudomonads can be
affiliated to group under CLP producing biotypes/biovars. Purification of CLP
(Viscosinamide) and characterization by HPLC analysis was carried out. Pot
culture experiments were conducted to test the performance of CLP producing Pf
strains in maize crop for testing their disease resistance. These biovars with
antibiotic properties are the potential targets for the disease management in
maize. CLPs in general receive considerable attention as potent antimicrobial
drugs.
Author(s) Details
Radhajeyalakshmi Raju
Maize Research Station,
Tamil Nadu Agricultural University, Vagarai-624613, Palani, Tamil Nadu, India.
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