Enzymatic Responses of Vigna radiata Seedlings under Biotic and Abiotic Stress | Chapter 13 | Theory and Applications of Microbiology and Biotechnology Vol. 2
Soil ecology is very fascinating due to
its composition in which microbial strains sometime play a marvelous role in
presence of several metals. The micronutrient metals are essential part of soil
provides help in germination while toxic when exceed to a certain limits. This
article aims to evaluate the cumulative effect of isolated bacterial strain of
Pseudomonas stutzeri with copper (Cu) as a bioactive element in seed
germination of four days old Vigna radiata. The germination of seeds also
monitored at varying concentrations of Cu as a micronutrient. An important
function of the enzyme lactate dehydrogenase (LDH) was observed in the shoots
of four days old seedlings of V. radiata, cultivated in a series of experiments
in the presence and absence of biotic and abiotic stresses simultaneously and
separately. It was observed that germination of the seedlings were inhibited in
presence of P. stutzeri separately may be due to the marked decrease in lipid
contents. Results showed high LDH activity and high glucose contents as
compared to the control ones indicated that high LDH activity was linked with
elevated energy demand to overcome the stress for germination of seeds.
Unexpectedly, germination of seeds was favored with increasing concentrations
of Cu (100 ppm) with P. stutzeri simultaneously, although both biotic and
abiotic seemed to be toxic separately. No microbial life at the highest
concentration of Cu showed that it was toxic to the bacterial strain, but
normal growth of seedlings suggested that dead mass of P. stutzeri was
effective for the adsorption of the Cu on their surface due to which Cu
mobility was checked recommencing the normal activity of LDH and glucose
contents that believed to be at the cost of lipid contents. A suitable
mechanism consistent with the finding has been proposed.
Author(s) Details
Prof. Dr. Rafia Azmat
Department of Chemistry,
University of Karachi, 75270, Karachi, Pakistan.
Aliya Hayat
Department of Microbiology,
Jinnah University for Women, 5C Nazimabad,
Karachi, 74600, Pakistan.
Prof. Dr. Farha Aziz
Department of Biochemistry,
Jinnah University for Women, 5C Nazimabad
Karachi, 74600, Pakistan.
Prof. Dr. Masooda Qadri
Department of Chemistry,
University of Karachi, 75270, Karachi, Pakistan.
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