In vivo Antioxidant Activity of Sida cordifolia Linn. in K2Cr2o7 Induced Oxidative Stress by Measurement of Reactive Oxygen Species Levels in Rats | Chapter 09 | New Insights into Disease and Pathogen Research Vol. 3
Ayurvedic textbooks ascribe many
therapeutic properties such as anti-inflammatory, antimicrobial and antioxidant
actions to Sida cordifolia Linn. which contains many polyphenols in its roots
and seeds. Evaluation of in vivo antioxidant
action in rats of the aqueous extract of Sida cordifolia Linn. roots has been
done by introducing potassium dichromate for creating acute and chronic
oxidative stress. The Folin- Ciocalteu reagent was utilized for assessing the
phenol content which was determined as 27.375 mg Gallic acid/gm. During the
acute toxicity tests using mice, no significant adverse signs or symptoms of
toxicity were noticed up to the dose of 1500 mg/kg and no mortality was
reported until this dosage. The Phosphomolybdate Assay using 3, 6 and 9 mg/ml
of research drug concentrations resulted in determination of the Total
Antioxidant Capacity that was found to be 15.40 ±9.13, 19.00 ±7.56 and 21.02
±8.42 gm equivalents of Ascorbic acid. The results of the d-ROMs test which
assessed the hydroperoxide levels clearly suggested that introduction of
research drug could significantly counter the negative effects of both acute
and chronic oxidative stress. Broadly similar findings were obtained by using
ELISA kit for assessing ROS values where concurrent administration of the
research drug resulted in very significant amelioration in respect of oxidative
stress conditions.
Author(s) Details
Dr. Mradu Gupta
Institute of Post Graduate
Ayurvedic Education and Research, 294/3/1, APC Road, Kolkata, India.
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