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Multidrug Resistant Escherichia coli Isolated from Asymptomatic School Going Children in Kibera Slum, Kenya | Chapter 07 | Theory and Applications of Microbiology and Biotechnology Vol. 2

Pathogenic Escherichia coli are of different types, currently grouped into six groups depending on the virulence gene(s) they possess. This study isolated pathogenic E. coli from 580 stool samples obtained in the month of August, 2016. The samples were obtained from asymptomatic school going children in one of the biggest urban slums in Kenya. Ten primary schools were randomly sampled and 40 to 80 stool samples collected from each school depending on the school population. Both gender and age were considered when sampling. Data obtained was analysed using single factor ANOVA to test association between school location and levels of infection with pathogenic bacteria. A total of 244 (17%) samples had E. coli. Out of these, 38 (6.5%) were shown to have one or a combination of the pathogenic genes, namely: ipaH, virF, st2, daaE, eae, aafII, stx1, bfp, lt and stII and were thus classified into seven groups. Of the pathogenic isolates 35 (21.2%) were multidrug resistant. There was an association between school location and the prevalence of pathogenic bacteria. In conclusion, asymptomatic school going children in the slum were found to be infected with multidrug resistant pathogenic E. coli.

Author(s) Details

Dr. N. J. Gitahi
Department of Public Health Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi, Kenya.

Dr. P. B. Gathura
Department of Public Health Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Nairobi, Kenya.

Professor  M. M. Gicheru
Department of Zoological Sciences, School of Pure and Applied Sciences, Kenyatta University, Nairobi, Kenya.

T. W. Githinji
School of Biological and Physical Sciences, University of Nairobi, Kenya.

Dr. A. Nordin
Department of Energy and Technology, Faculty of Natural Resources and Agricultural Sciences, Swedish University of Agricultural Sciences, Sweden.

View Book: http://bp.bookpi.org/index.php/bpi/catalog/book/107

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