Skip to main content

The Use of Streptomyces capoamus Isolated from Caatinga in the Production and Characterization of Microbial Endoglucanase | Chapter 05 | Advances and Trends in Biotechnology and Genetics Vol. 3

Cellulases are hydrolases of great importance to industries, especially due to their ability to produce ethanol via hydrolysis of cellulolytic materials. Actinomycetes are the producers of these enzymes, particularly the genus Streptomyces sp. The present study is the first report on the production and characterization of cellulolytic complex secreted by Streptomyces capoamus, isolated from the rhizosphere soil of Caatinga. In selecting the microbial producers of cellulolytic complex in qualitative tests, 171 x micro organism showed the most expressive enzymatic index. Regarding the production time of the complex, fermentation was done for 7 days, with aliquots being taken every 24 h. Peak production was obtained during 48 h fermentation. It was done at 37°C and under an agitation of 180 rpm. It was noted also that the 171x micro-organism produced the enzyme in greater quantity. The experiment was done with the most significant actinomycetes (171x), optimal substrate concentration (carboximeticellulose), cultivation temperature and pH of initial output. The results showed that a higher cellulolytic complex was obtained with 2% substrate, 45°C temperature and initial pH 4.0. The microorganism was identified at genus level by microculture method; and with molecular identification method, it was identified as S. capoamus UFPEDA-3410. In optimal culture conditions, this strain produced 0.309 U/mL cellulose, a good production for a thermostable endoglucanase stable in a broad range of pH and stable temperature. It has potential applications in a wide range of industries. Industrial processes are generally carried out at elevated temperatures. Therefore enzymes with a high optima temperature and stability are desired for such applications.

Author(s) Details

Rafael Lopes e Oliveira
Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
Multidisciplinary Support Center, Federal University of Amazonas, CEP 69077-000, Manaus-AM, Brazil.

Camila Beatriz Atanásio Borba
Department of Antibiotics, Biological Sciences Center, Federal University of Pernambuco, Brazil, CEP 50670-901, Recife-PE, Brazil.

Sergio Duvoisin Junior
Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.

Patricia Melchionna Albuquerque
Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
Graduate Program in Biotechnology and Natural Resources, School of Health Sciences, State University of Amazonas, CEP 69065-001, Manaus-AM, Brazil.

Norma Buarque de Gusmão
Department of Antibiotics, Biological Sciences Center, Federal University of Pernambuco, Brazil, CEP 50670-901, Recife-PE, Brazil.

Edmar Vaz de Andrade
Multidisciplinary Support Center, Federal University of Amazonas, CEP 69077-000, Manaus-AM, Brazil.

Leonor Alves de Oliveira da Silva
Multidisciplinary Support Center, Federal University of Amazonas, CEP 69077-000, Manaus-AM, Brazil.
Department of Antibiotics, Biological Sciences Center, Federal University of Pernambuco, Brazil, CEP 50670-901, Recife-PE, Brazil.

View Books: http://bp.bookpi.org/index.php/bpi/catalog/book/111

Comments

Popular posts from this blog

Empowerment and Leader Member Exchange towards Organizational Citizenship Behavior Readiness among Government Workforce in Malaysia | Book Publisher International

  State agencies are among the most significant entities that represent the public's needs, and their programmes are essential to a state's and the nation's economic growth and development. State agencies have been rife by public concerns for years, as demonstrated by the Public Complaints Bureau, as problems of inefficiency in service quality and delivery have yet to meet the public's standards. With the support of 15 employees from state agencies on Malaysia's east coast, this study aims to achieve three targets. The first and second objectives are to investigate the ties between leader-member exchange (LMX) and OCB empowerment. The final aim is to define the most significant factors that affect OCB willingness. A total of 288 people took part in the survey, which was conducted using a cluster sampling process. The survey items had achieved the necessary reliability, according to the pilot test results, and some sub-dimensions were renamed after factor analysis.

Re-asserting Cultural Perspectives: Old People and New Ideas in Bole Butake’s Lake God and The Survivors and Sankie Maimo’s Succession in Sarkov | Chapter 10 | Perspectives of Arts and Social Studies Vol. 2

African cultures have undergone transformations from the colonial period to the present, often to the detriment of its cultural evolution and within the larger global community. As it were, colonial education subordinated African communalism and created in its place an anti-African spirit evident in the assimilation of western values. This did not only end up in a kind of cultural betrayal, but also posed as a serious threat to the dignity and identity of the people. Those who fall prey to this kind of cultural imperialism are the young people who are often irrationally carried away by western fashion and modes that they tend to neglect and/or forget their cultural ways of life as they join the race of “progress.” This situation has given rise to a conscious effort by creative writers to re-assert and protect African values while at the same time liberating themselves from the longstanding western effort at suppressing, controlling and dominating their thoughts particularly through n

The Utilization of Agro-Waste: A Nanobiotechnology Point of View | Chapter 10 | Recent Advances in Biological Research Vol. 5

Aims: To review the utilization of agro-waste in the eco-friendly synthesis of nanoparticles and their biomedical, catalytic and industrial applications. Study Design: A review. Place and Duration of Study: This review was carried out in the interim of three weeks exploiting all relevant data, literature and publications where necessary. Methodology: Profound gathering of literatures/publications and reviews were employed with all carefulness and professional courtesy; enough useful information were gathered over time. The introduction emphasizes the dawn of science and technology, when great ideas were still latent later leading to the advent of history changing innovations like the completion of human genome and then the birth of nanotechnology. Although, nanotechnology had been known decades been popularized by Richard Feynman in his talks in the year 1959; and in this present dispensation, nanotechnology has been a solution to many intricate challenges/ t