Skip to main content

The Use of Streptomyces capoamus Isolated from Caatinga in the Production and Characterization of Microbial Endoglucanase | Chapter 05 | Advances and Trends in Biotechnology and Genetics Vol. 3

Cellulases are hydrolases of great importance to industries, especially due to their ability to produce ethanol via hydrolysis of cellulolytic materials. Actinomycetes are the producers of these enzymes, particularly the genus Streptomyces sp. The present study is the first report on the production and characterization of cellulolytic complex secreted by Streptomyces capoamus, isolated from the rhizosphere soil of Caatinga. In selecting the microbial producers of cellulolytic complex in qualitative tests, 171 x micro organism showed the most expressive enzymatic index. Regarding the production time of the complex, fermentation was done for 7 days, with aliquots being taken every 24 h. Peak production was obtained during 48 h fermentation. It was done at 37°C and under an agitation of 180 rpm. It was noted also that the 171x micro-organism produced the enzyme in greater quantity. The experiment was done with the most significant actinomycetes (171x), optimal substrate concentration (carboximeticellulose), cultivation temperature and pH of initial output. The results showed that a higher cellulolytic complex was obtained with 2% substrate, 45°C temperature and initial pH 4.0. The microorganism was identified at genus level by microculture method; and with molecular identification method, it was identified as S. capoamus UFPEDA-3410. In optimal culture conditions, this strain produced 0.309 U/mL cellulose, a good production for a thermostable endoglucanase stable in a broad range of pH and stable temperature. It has potential applications in a wide range of industries. Industrial processes are generally carried out at elevated temperatures. Therefore enzymes with a high optima temperature and stability are desired for such applications.

Author(s) Details

Rafael Lopes e Oliveira
Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
Multidisciplinary Support Center, Federal University of Amazonas, CEP 69077-000, Manaus-AM, Brazil.

Camila Beatriz Atanásio Borba
Department of Antibiotics, Biological Sciences Center, Federal University of Pernambuco, Brazil, CEP 50670-901, Recife-PE, Brazil.

Sergio Duvoisin Junior
Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.

Patricia Melchionna Albuquerque
Laboratory of Applied Chemistry and Technology, Chemical Engineering Course, School of Technology, State University of Amazonas, CEP 69050-020, Manaus-AM, Brazil.
Graduate Program in Biotechnology and Natural Resources, School of Health Sciences, State University of Amazonas, CEP 69065-001, Manaus-AM, Brazil.

Norma Buarque de Gusmão
Department of Antibiotics, Biological Sciences Center, Federal University of Pernambuco, Brazil, CEP 50670-901, Recife-PE, Brazil.

Edmar Vaz de Andrade
Multidisciplinary Support Center, Federal University of Amazonas, CEP 69077-000, Manaus-AM, Brazil.

Leonor Alves de Oliveira da Silva
Multidisciplinary Support Center, Federal University of Amazonas, CEP 69077-000, Manaus-AM, Brazil.
Department of Antibiotics, Biological Sciences Center, Federal University of Pernambuco, Brazil, CEP 50670-901, Recife-PE, Brazil.

View Books: http://bp.bookpi.org/index.php/bpi/catalog/book/111

Comments

Popular posts from this blog

A Brief Study of Middleware Technologies: Programming Applications and Management Systems | Chapter 15 | Novel Research Aspects in Mathematical and Computer Science Vol. 1

  Many platforms, services, applications, hardware, and operating systems are connected through the middleware layer. Because the middleware layer abstracts much low-level complexity and makes applications and software systems portable, it allows disparate systems to interface and function together in harmony. Middleware technologies enable software engineers to swiftly construct software systems and applications, allowing developers to focus on more important tasks. This chapter examines several types of middleware systems and discusses middleware capabilities, middleware operation, middleware's function in cloud-based systems, and the best middleware platforms to use. Middleware systems are widely utilised and can be found in practically any software system or application. Middleware programmes provide as a link between many sorts of systems and protocols. They serve as a mechanism for various systems. To successfully exchange information, it runs on a variety of operating system

A Prospective Study about Safety and Efficacy of Perioperative Lidocaine Infusion | Chapter 09 | New Horizons in Medicine and Medical Research Vol. 8

 Opioids cause clinically significant side effects such as respiratory depression, immunosuppression, muscle rigidity, negative inotropism, nausea, vomiting, hyperalgesia, urine retention, postoperative ileus, and drowsiness. Perioperative opioids are a major contributor to the United States' and other countries' opioid epidemics. Non-opioid analgesics, particularly lidocaine, are becoming more common for perioperative use as a result of this. A total of 185 adult patients were randomly assigned to one of two groups: control group I (105 patients) [fentanyl group] or group ii (80 patients) [opioid-free anaesthesia group]. Lidocaine 1.5 mg/kg bolus followed by 1.5 mg/kg/h infusion intraoperatively, and 1.5-2 mg/kg/h infusion for 2-8 hours postoperatively were given to patients in both groups at anaesthetic induction. Intraoperatively, both groups received analgesic adjuvants such as diclofenac 75 mg, paracetamol 1 gm, and mgso4 30-50 mg/kg. If the mean arterial pressure (map)

Scientific values of ECA Ligation | Chapter 06 | New Horizons in Medicine and Medical Research Vol. 8

  Previously, carotid artery ligation was done as an emergency procedure to stop severe haemorrhages in the head and neck. Despite this, it was abandoned because to high patient mortality and morbidity. However, ECA ligation, a more selective branch of the carotid artery, has been popular for the same goal in recent decades due to the lack of or modest postsurgical problems compared to Transarterial embolization (TAE). However, a good surge seal pack, especially in the maxilla, is occasionally required to regulate both collateral circulation and backflow of blood from the internal carotid arteries distal to the carotid arteries (ICA). ECA ligation, on the other hand, might be a superior alternative for controlling life-threatening uncontrolled bleeding in this area. Author(S) Details Jachmen Sultana Department of Oral and Maxillofacial Surgery, Dhaka Dental College and Hospital, Bangladesh. Abul Bashar Department of Paediatrics, Comilla Medical College and Hospital, Bangladesh.