Skip to main content

Thermophilic Bacteria: Novel Polymers for Biotechnological Applications | Chapter 01 | Advances and Trends in Biotechnology and Genetics Vol. 3

Aims: We analyzed thermotolerant bacterial isolates of thermophiles from various regions in South East coasts of India. They were analyzed for Fatty Acid Methyl Ester (FAME) for identification and classification.

Study Design: A total of forty three (9.6%) thermotolerant bacterial isolates of thermophiles were secluded out of 444 isolates obtained from water samples from the Adyar River, Marina coast, Muttu Kadu Backwaters, Kovalam coast (India) and a prawn hatchery. In addition, a novel thermotolerant Gram positive Bacillus type bacterium from the pelletized feed of fish was examined. A total of 18 thermotolerant bacteria were analyzed for Fatty Acid Methyl Ester (FAME).

Results: Amongst 134 isolates that grew on E. coli FAGI agar, 23 (17%) were thermotolerant when tested at 15 psi for 30 min. Of the 72 isolates that grew on Staphylococcus Baird Parker agar 10 (13%) were thermotolerant, whilst among the 128 isolates that grew on Salmonella agar ÖNÖZ 10 (8%) were thermotolerant. In contrast, none of the 110 isolates that grew on TCBS agar (Vibrio sp.) exhibited thermotolerance, although they were isolated from the same regions as the other bacteria. The optimal temperature for the growth of most thermophiles was 45°C, except for 9 isolates. Of the latter, 7 grew at 50°C, one at 52°C and one at 55ºC, respectively.There were some extremely thermotolerant bacterial isolates that exhibited consistency in profuse growth at 15 psi (121°C) for 30 min. Other isolates showed inconsistency in growth after autoclaving the cultures at 15 psi (121°C) for 30 min, and these bacteria showed development of endospores. Isolates of thermotolerant bacteria that grew on Staphylococcus Baird Parker agar and Salmonella agar ÖNÖZ were predominantly antibiotic resistant when compared with those bacterial isolates that grew on E. coli FAGI agar. A major 43 kDa toxin-protein as well as 15 other minor protein subunits was detected in the thermotolerant Bacillus sp. isolate. The proteins were toxic and caused mortality to goldfish Cyprin macrophthalmus. The thermotolerant bacteria were identified as B. cereus, Bacillus subtilis, B. pumilus, B. laevolacticus), B. alcalophilus, Kurthiasibirica, Geobacillus stearothermophilus, Staphylococcus schleiferi and S. gallinarum,

Conclusions: This study documents 43 thermotolerant bacterial isolates from water samples and a Bacillus-type bacterium from fish feed in Chennai region, India and these bacterial isolates offer the potential for exploitation in biotechnological applications.

Author(s) Details

Palaniappan Ramasamy
Research and Development Wing, Sree Balaji Medical College and Hospital, BIHER (Bharath University), Chromepet, Tamil Nadu, Chennai-600044, India.

Aruna Sharmili
Department of Biotechnology, Stella Mary’s College, Tamil Nadu, Chennai-600032, India.

View Books: http://bp.bookpi.org/index.php/bpi/catalog/book/111
Labels:

Comments

Post a Comment

Popular posts from this blog

A Prospective Study about Safety and Efficacy of Perioperative Lidocaine Infusion | Chapter 09 | New Horizons in Medicine and Medical Research Vol. 8

 Opioids cause clinically significant side effects such as respiratory depression, immunosuppression, muscle rigidity, negative inotropism, nausea, vomiting, hyperalgesia, urine retention, postoperative ileus, and drowsiness. Perioperative opioids are a major contributor to the United States' and other countries' opioid epidemics. Non-opioid analgesics, particularly lidocaine, are becoming more common for perioperative use as a result of this. A total of 185 adult patients were randomly assigned to one of two groups: control group I (105 patients) [fentanyl group] or group ii (80 patients) [opioid-free anaesthesia group]. Lidocaine 1.5 mg/kg bolus followed by 1.5 mg/kg/h infusion intraoperatively, and 1.5-2 mg/kg/h infusion for 2-8 hours postoperatively were given to patients in both groups at anaesthetic induction. Intraoperatively, both groups received analgesic adjuvants such as diclofenac 75 mg, paracetamol 1 gm, and mgso4 30-50 mg/kg. If the mean arterial pressure (map)
Post a Comment
Read more

A Brief Study of Middleware Technologies: Programming Applications and Management Systems | Chapter 15 | Novel Research Aspects in Mathematical and Computer Science Vol. 1

  Many platforms, services, applications, hardware, and operating systems are connected through the middleware layer. Because the middleware layer abstracts much low-level complexity and makes applications and software systems portable, it allows disparate systems to interface and function together in harmony. Middleware technologies enable software engineers to swiftly construct software systems and applications, allowing developers to focus on more important tasks. This chapter examines several types of middleware systems and discusses middleware capabilities, middleware operation, middleware's function in cloud-based systems, and the best middleware platforms to use. Middleware systems are widely utilised and can be found in practically any software system or application. Middleware programmes provide as a link between many sorts of systems and protocols. They serve as a mechanism for various systems. To successfully exchange information, it runs on a variety of operating system
Post a Comment
Read more

Patients’ Perspective of Acute Post-operative Pain Management: A Multicentre Survey of Tertiary Hospitals in Maharashtra, India | Chapter 08 | New Horizons in Medicine and Medical Research Vol. 8

 When postoperative pain is adequately controlled, patients' satisfaction and patient-related outcomes (PROs) increase. Understanding the patients' perspective is crucial since it supports in the formulation of improvement strategies. Because wrong attitudes and assumptions might block pain alleviation, patients' attitudes and beliefs are critical. As a result, a multicenter study of patients' attitudes, beliefs, experiences, and satisfaction levels with acute postoperative pain management was done in Maharashtra's tertiary hospitals. In addition, the responses were examined to evaluate if the Acute Pain Service (APS) resulted in improved patient outcomes and satisfaction. A 13-item questionnaire adapted from previous studies was used to capture patients' experiences with postoperative pain treatment. The responses of 179 patients are included in the study. The findings revealed that 91.6 percent of patients experienced postoperative pain, with 75.5 percent
Post a Comment
Read more