Time Kill-kinetics Antibacterial Study of Acacia nilotica | Chapter 06 | Theory and Applications of Microbiology and Biotechnology Vol. 2
In vitro time-kill kinetics
antibacterial study of Acacia nilotica was assessed against Pseudomonas
aeruginosa, Escherichia coli and Staphylococcus aureus determined by plate
count technique and analyzed by percentage and log reduction. All test
organisms were susceptible to the aqueous methanolic extract. The minimum
inhibitory concentration ranged between 0.5 and 1 mg, while minimum
bactericidal concentration ranged between ≥1 and ≥2 mg/ml. Average log
reductions in viable cell counts for the extract ranged between 0.18log10
and 0.35log10 cfu/ml for P. aeruginosa, 0.27log10 and
1.95log10 cfu/ml for S. aureus and 0.27log10 and 0.45log10
cfu/ml for E. coli after 10 h interaction at 0.5x MIC and 1x MIC. Most of the
extracts were rapidly bactericidal at 2x MIC achieving a complete elimination
of most of the test organisms within 12 h exposure. A good correlation was found
between the killing curves and the MIC of A. nilotica against the test
organisms.
Author(s) Details
P. Oladosu
National Institute for
Pharmaceutical Research and Development, Idu Industrial Area, P.M.B. 21, Garki,
Abuja, Nigeria and Department of Biology, Faculty of Science, University of
Abuja, Gwagwalada, Abuja, Nigeria.
N. R. Isu
Department of Biology,
Faculty of Science, University of Abuja, Gwagwalada, Abuja, Nigeria.
K. Ibrahim
National Institute for
Pharmaceutical Research and Development, Idu Industrial Area, P.M.B. 21, Garki,
Abuja, Nigeria.
P. Okolo
National Institute for
Pharmaceutical Research and Development, Idu Industrial Area, P.M.B. 21, Garki,
Abuja, Nigeria.
D. K. Oladepo
National Institute for
Pharmaceutical Research and Development, Idu Industrial Area, P.M.B. 21, Garki,
Abuja, Nigeria.
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