Skip to main content

Usefulness of Commercial Rapid Test Kits as an Effective Diagnostic Tool of Dengue Virus Infection in a Low Resource Setting | Chapter 09 | Theory and Applications of Microbiology and Biotechnology Vol. 2

Rapid immuno-chromatographic tests (ICTs) or diagnostic kits that are commercially available were evaluated for their sensitivity, specificity, cost and turnaround time (TAT) results with Dengue IgM/IgG capture enzyme linked immunosorbent assays (ELISA) as the standard test, in blood samples from a cross-section of individuals with clinical features suggestive of dengue fever attending health care facilities in Trinidad and Tobago. Blood samples taken from 100 consented participants were analyzed using the two rapid ICTs (SD Bioline Dengue Duo NS1/IgM/IgG and Panbio Dengue Duo Cassette) and compared with the Dengue IgM/IgG capture ELISAs. Standardized questionnaire was used to obtain bio and epidemiological data of the participants. The laboratory evaluation also assessed the TAT to complete the tests as well as the cost for each test method. The laboratory analysis on a given number (n=93) revealed that the SD Bioline was more sensitive (39.9%) than the Panbio (22.1%; p=0.005), and specificities for both were 100%. The SD Bioline includes an extra biomarker test with the same TAT and differs in cost by USD$ 1.14 as opposed to the Panbio. The ELISA has a cost of USD$ 8.07 and despite its longer TAT, it has the advantage of running more samples (1 vs 96) at a given time. While SD Bioline may be the better choice with a higher sensitivity, dengue ELISAs should also be favourably considered as an option for diagnostic purposes. In a resource strapped setting like the laboratories in Trinidad and Tobago, the ELISA should be preferred because its sensitivity and specificity were higher than the Panbio and SD Bioline kits. Besides, more samples were tested giving an effective TAT for amounts of samples completed despite a higher cost.

Author(s) Details

Professor Patrick Eberechi Akpaka, MBBS, DM
Department of Paraclinical Sciences, Unit of Pathology and Microbiology, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.

Kadia Kallap, BSc, MSc
Department of Paraclinical Sciences, Unit of Pathology and Microbiology, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.

Dr. Chandrashekhar Unakal, PhD
Department of Paraclinical Sciences, Unit of Pathology and Microbiology, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.

Dr. Arvind Kurhade, MBBS, MD
Department of Paraclinical Sciences, Unit of Pathology and Microbiology, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.

View Book: http://bp.bookpi.org/index.php/bpi/catalog/book/107
Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

Occipital Dermal Sinus Tract Causing Craniospinal Infection: A Review | Chapter 13 | New Visions in Medicine and Medical Science Vol. 4

  Dermal sinus is a rare congenital condition characterized by a pathological tract connecting the skin to deeper tissues of the central nervous system, potentially leading to severe infectious complications. It arises from a failure in the separation of ectodermal layers during early gestation. Diagnosing dermal sinus tract in newborns requires a careful physical examination, focusing on midline dimples in the occipital region associated with cutaneous abnormalities like hairy nevus or hyperpigmentation. The presence of drainage, abnormal hair distribution, or localized swelling may indicate a sinus tract. Regular examination for dimples or sinuses is recommended for infants and children with recurrent meningitis or infections. Early detection is crucial to prevent severe complications like meningitis, with Staphylococcus aureus being a common causative organism. Neuroradiological studies, including computed tomography (CT) scan and magnetic resonance imaging (MRI), with histopath...
Post a Comment
Read more

Alkali Element Modification of Glucose Molecules as a Method to Dissolve Cancer Cells | Chapter 12 | New Visions in Medicine and Medical Science Vol. 4

  The present study highlights about alkali element modification of glucose molecules as a method to dissolve cancer cells. The central regulation of the mechanisms governing cell proliferation has little effect on cancer cells. Cancer cells are entirely independent of the central command and divide and proliferate on their own, making it challenging to activate their response mechanism. Precisely, this is the reason why they are at risk to the health of humans and/or any biological entities. Instead of trying to reconnect the central command of the growth control mechanism to cancer cells that are already out of the range, we present a method of using the cancer cell’s own irresponsive and uncontrolled growth mechanism to their disadvantage and destroy the cancer cells. We found that this is achievable in an atomic/molecular level study of the glucose molecule, which is the primary food source used for growth and energy generation by all cells in the body, including the cancer cel...
Post a Comment
Read more