Caffeine and Phenolic Compounds in Cola nitida (Vent.) Schott and Endl and Garcinia kola Heckel Grown in Côte d’Ivoire | Chapter 02 | Emerging Issues in Science and Technology Vol. 2
Kola nuts are usually eaten fresh and
represent a substantial source of income for many households and certain public
authorities. Their importance lies in their content of some secondary
metabolites of interest used in industries for the production of energy drinks
and pharmaceuticals. The aim of this study is to reveal and determine the
content of polyphenols (flavonoids in particular) and caffeine in Côte d’Ivoire
kola nuts. According to their phenotypical characteristics, four lots of nuts
were collected (Red Cola nitida (RCN); White Cola nitida (WCN); Purple Cola
nitida (PCN) and Garcinia kola (WGK)). After drying, different extractions were
carried out using several solvents: water, acidified water (0.01N citric acid),
methanol, ethanol, acetone 60% and methanol/acetic acid 1% solution.
Phytochemical screening showed that kola nuts contain antioxidants such as
flavonoids, tannins and alkaloids in varying proportions according to the
species. Spectrophotometric analysis revealed that total polyphenols and
flavonoids are important in C. nitida nuts compared to those of G. kola.
Phenolic contents are 26.76±0.54; 23.08±1.06; 17.06±1.03 and 14.90±0.64 mg/g FW
for WCN, RCN, PCN and WGK, respectively. As for flavonoids, levels are
803.03±14.48, 697.13±12.76, 647.76±21.16 and 355.74±17.03 mg/kg FW for WCN,
RCN, PCN and WGK respectively. It noted that acetone 60% is the efficient
solvent for extraction. Statistical analyzes indicate the significant influence
at P=.05 of solvent and nuts morphotype on the level of extractable for total
polyphenols and flavonoids. Concerning caffeine, reversed-phase HPLC analysis
indicates high content of caffeine in C. nitida extracts with a slight
predominance for RCN (10.81±0.06 g/kg FW). We note a small amount of this
metabolite (80.08 ±2.91 mg/kg FW) in G. kola. The mean intakes of nutrients are
3.99 and 1.22 g of polyphenol/day for an equivalent daily intake of 0.6 g (cola
amount consumed per day in West Africa) and 183 g (amount of fruit consumed per
day). About caffeine, intakes are 4.31 and 1.31 g/day for 0.6 and 183 g
respectively. These findings indicate that kola seeds are enriched in important
compound and can be used as a possible source of antioxidant for African
population’s customs and European industries.
Author(s) Details
Y. Nyamien
Laboratory of Biochemistry
and Food Science, UFR Biosciences, Felix HOUPHOUËT-BOIGNY University of
Abidjan, 22 P.O 582 Abidjan 22, Côte d'Ivoire and Institute of Agropastoral
Management, Peleforo Gon COULIBALY University,P.O Box 1328 Korhogo, Côte
d'Ivoire.
F. Adje
Laboratory of Water
Chemistry and Natural substances, DFR GCAA, Felix HOUPHOUËT-BOIGNY National
Institute, P.O 1093 Yamoussoukro, Côte d’Ivoire.
F. Niamke
Laboratory of Water
Chemistry and Natural substances, DFR GCAA, Felix HOUPHOUËT-BOIGNY National
Institute, P.O 1093 Yamoussoukro, Côte d’Ivoire.
O. Chatigre
Laboratory of Biochemistry
and Food Science, UFR Biosciences, Felix HOUPHOUËT-BOIGNY University of
Abidjan, 22 P.O 582 Abidjan 22, Côte d'Ivoire.
A. Adima
Laboratory of Water
Chemistry and Natural substances, DFR GCAA, Felix HOUPHOUËT-BOIGNY National
Institute, P.O 1093 Yamoussoukro, Côte d’Ivoire.
G. H. Biego
Laboratory of Biochemistry
and Food Science, UFR Biosciences, Felix HOUPHOUËT-BOIGNY University of
Abidjan, 22 P.O 582 Abidjan 22, Côte d'Ivoire.
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