Effect of Light and Temperature through Poly Film Covers on Anthocyanin Content in Rose Cut Flower | Chapter 06 | Modern Research in Botany Vol. 1
Quality is the most important attribute
in rose cut flowers for both export and domestic market. Quality in cut flowers
may be defined by many attributes however, among the most important is colour.
A group of pigments commonly known as anthocyanins determine colour in plants. Anthocyanins
play a significant role by ameliorate the effect of high irradiance in plants
under stressful environment. They also play a key role in delaying senescence
hence enhancing the cut flower vase life. Despite the advantages anthocyanins
are affected by the preharvest conditions mainly light and temperature
interfering with their stability. An experiment was set up to investigate the
effect of light and temperature through selected coloured poly film covers on
rose petal anthocyanin content. The greenhouse structure was covered by poly
films of different colours that were compartmentalized i.e. UV-A clear, IR504
with yellow tint and UV-A 205/N with green tint replicated three times. Two
rose cultivars Red calypso and Furiosa were established and maintained, upon
maturity the flower heads were plucked and oven dried at 60°C to constant
weight. 5 g of the crushed petals was used in anthocyanin extraction. The
anthocyanins were extracted and quantified in comparison with commercial
standards using HPLC machine. The data obtained from the chromatogram as peak
areas was subjected to analysis of variance (ANOVA) using SAS statistical
package (SAS Inst., Inc., Cary, NC) at P = .05. Where there were treatment
differences, mean separation was done using Tukey’s procedure. Poly films
significantly affected the quantity and quality of anthocyanin accumulation in
rose petals. Cyanidin 3-0-glucoside was the most prevalent anthocyanin across
all poly film covers and it was noted to be high under the UV-A 205/N
(110.95±8.26 µg _ 5 g–1 DW) and IR504 (109.69±8.26 µg _ 5 g–1 DW) compared to
UV-A clear (84.56±8.26 µg _ 5 g–1 DW). The quantity of anthocyanins was low
under the UV-A clear poly film that was characterized by high light
transmission and day temperature. Combination of high irradiance and
temperature affect the quality and quantity of anthocyanin in rose cut flowers.
Author(s) Details
Dr. G. Oloo-Abucheli
Department of Plant
Sciences, Chuka University, P.O.Box 109-60400, Chuka, Kenya and Department of
Crops, Horticulture and Soil Sciences, Egerton University, P.O.Box 536,
Egerton, Kenya.
Prof. J. N. Aguyoh
Department of Agriculture
and Environmental Studies, Rongo University College, P.O.Box 1023-40404, Rongo,
Kenya.
G. Liu
Department of Crops,
Horticulture and Soil Sciences, Egerton University, P.O.Box 536, Egerton,
Kenya.
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