In vitro Propagation of a Medicinal Plant Adhatoda vasica Nees by Shoot Bud Culture | Chapter 14 | Modern Research in Botany Vol. 1
Mass propagation of plant species
through in vitro culture is one of the useful and most successful examples of
commercial application of plant tissue culture technology. Recently, much
progress has been done in this technology for regenerating medicinal plants. In
present study an efficient protocol is devised for a rapid in vitro propagation
through shoot bud culture of a valuable medicinal plant Adhatoda vasica. In
present investigation the proliferating auxiliary shoot cultures were
established on MS medium and Gamborg B5 medium supplemented with different
concentrations of BAP, NAA, kinetin and 2,4-D using nodal explants from the
field grown mature healthy plant of Adhatoda vasica. After 30 days of culture
raised from nodal explants of Adhatoda vasica, maximum number of shoots was
produced, on MS medium supplemented with 1.0 mg/l BAP. These explants had
developed more than two shoots per nodes, while in other concentration of Kn,
NAA and 2,4-D developed either two or less than two shoots/explants. Highest
frequency of shoot formation and maximum number of shoots per explants were
obtained on MS medium supplemented with 1 mg/I IBA.
Author(s) Details
Dr. P. Soni
Department of Biotechnology,
Spcas College, Raipur, Chhattisgarh, India.
Dr. A. N. Bahadur
[Professor]
Department of Botany,
Government E. Raghavendra Rao Postgraduate Science College, Bilaspur, Chhattisgarh, India.
Dr. V. K. Kanungo
[Assistant Professor]
Government Nagarjuna Post
Graduate College of Science, Chhattisgarh, India.
Dr. U. Tiwari [Asst.
Professor]
Department of Botany,
Government E. Raghavendra Rao Postgraduate Science College, Bilaspur, Chhattisgarh, India.
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