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Modification of Surface Structure and Crystallinity of Water Hyacinth (Eichhornia crassipes) Following Recombinant α-L-arabinofuranosidase (ABFA) Treatment | Chapter 10 | Current Research in Agriculture and Horticulture Vol. 1

This research was aimed to investigate the modification of surface structure and crystallinity of water hyacinth after recombinant α-L-arabinofuranosidase treatment. The process of water hyacinth hydrolysis was optimized first, by determination of the most optimal intracellular enzyme (P) and extracellular enzyme (S) mixture ratio, and its incubation time. The optimum of water hyacinth hydrolysis process was achieved at ratio P:S = 1:2, after 8 h incubation. The surface structure of water hyacinth after recombinant α-L-arabinofuranosidase treatment was analyzed by scanning electron microscope (SEM) and X-ray diffraction (XRD), using untreated water hyacinth as control. This research showed that enzymatic hydrolysis damaged the surface structure of water hyacinth and changed its crystallinity. Similar result also indicated that, the α-L-arabinofuranosidase could also damaged the surface structure of rice straw at the optimum ratio of enzyme mixture was 1:1, also after 8 h incubation. The enzyme used in this research can be applied further for bio-bleaching of paper pulp and animal feed stock, while the sugar product can be applied for bioethanol production.

Author(s) Details

Anita Kurniati
Proteomic Laboratory, Research Center for Bio-Molecule engineering, Universitas Airlangga, Campus C Mulyorejo, Surabaya, East Java 60115, Indonesia.

Handoko Darmokoesoemo
Department of Chemistry, Faculty of Science and Technology, Univeristas Airlangga, Campus C Mulyorejo, Surabaya, East Java 60115, Indonesia.

N. N. T. Puspaningsih
Proteomic Laboratory, Research Center for Bio-Molecule engineering, Universitas Airlangga, Campus C Mulyorejo, Surabaya, East Java 60115, Indonesia.
Department of Chemistry, Faculty of Science and Technology, Univeristas Airlangga, Campus C Mulyorejo, Surabaya, East Java 60115, Indonesia.

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