Physiological Panel of Some Feed Additives for Turkey Toms | Chapter 03 | Research and Development in Agricultural Sciences Vol. 2
Aims:
To find the effect of basil and thyme (medicinal plants) and some enzymes
(kemzyme and zymogen) as feed additives on some productive performance, some
metabolic parameters, integrity and functionality of gastrointestinal tract.
Study Design: Forty - eight male
turkey toms (strain, Big 6), 3 months old were used with an average body weight
5.5 kg. All turkeys were apparently active and healthy. The turkey toms were raised on floor covered
with dry wood shaving, which was used in all partition, its thickness is about
10-15 cm and renewed every 3 days till the end of the experiment. The house was
divided into 8 partitions, each partition was (240 cm X 180 cm) providing
enough surface area for each turkey. In each partition there were plastic
feeders and drinkers of 20 L capacity, it was provided with identifying card to
record all required information. Food and water were available ad-libitum
through the period of experiment. The house was well ventilated by natural
windows. Light was turned off (one hour in 24 hours) as recommended by [1].
Turkey was kept under hygienic condition and as far as possible from any
discomfort stimuli. Feeding Regimen;
Basal ration were formulated to cover the nutrient requirements of growing
turkey according to National Research Center [2]. Toms were randomly divided
into eight groups (6 toms/each). The control group (C) was fed a basal diet
without feed supplementation. Group(B) was supplemented with basil (3 g
basil/kg diet) group (T) was fed thyme (2 g thyme/kg diet), group (TB) was fed
a mixture of basil and thyme (3 g basil + 2 g thyme kg diet), group (Z) was fed
zymogen (1 ml/4 liter water); and group (K) was supplemented with kemzyme (0.5
g/kg diet), group (BTK) was fed a mixture of basil, thyme and kemzyme, the last
group was fed with diet supplemented with basil, thyme and zymogen (BTZ).
Place and Duration of Study: The
present study was carried out in animal care center, faculty of veterinary medicine, Cairo University.
During the period from March till May 2015. The experiment lasted for 10 weeks.
Methodology: Body weight gain, feed
conversion ratio was measured at the end of 10th week of experiment. Blood
samples were taken to measure some biochemical & hormonal parameters, at
the same time some tissues for morphological and molecular studies were
prepared. Tissue samples were taken from duodenum. They were stained with
hematoxylin and eosin (H&E) stain for measuring the length of villi and the
depth of crypts using A computerized microscopic image analyzer attached for
full HD microscopic camera (Leica Microsystems, Germany was used to determine
the histomorphometry parameters [3]. The other part of the collected tissues
was prepared as homogenate for total DNA& RNA purification according to [4]
using the QIAamp Mini Kit.
Results: Toms fed with either basil,
thyme or kemzyme (BT) had significantly (p < 0.05) heaviest body gain than
the control, (Z) or (BTZ). Significant increase occurred of the dressing
percent (DP%) in group and (BT) compared to the groups (T, Z, BTK and BTZ).
Supplementation with (K) significantly decreases the serum total lipids than
the (C) group and all supplemented groups at P < 0.05. Serum cholesterol
levels of the groups (T), (BT) and (Z) recorded a significant increase than
those of groups (C), (K), (BTK) and (BTZ). Meanwhile; triglyceride levels
revealed
significant decrease in control group at (p < 0.05) than all other groups.
All experimental groups recorded no differences in both serum protein and
albumin. Although there were an increased levels of serum AST of groups (T) and
(Z) compared to control and other groups, meanwhile; (T) and (Z) groups
revealed the lowest level of serum ALT.
Concerning the antioxidant parameters, results reviewed that (T), (BT), (BTK)
and (BTZ) had a higher level of MDA activity than the (B), (K) and (Z)
supplemented groups illustrate no difference versus the serum (MDA) activity of
the (C) group. Serum SOD activity revealed no differences within all groups.
Serum TAC recorded significant increase in all supplemented groups compared to
their level in control group.
Studies of intestinal integrity; morphometry studies indicated that the villous
height increased in groups (T), (Z) and (BTK) with a higher villous to crypt
ratio and goblet cell numbers of group supplemented with (BTK). Moreover, the
villous width revealed a significant increase in the (C) and (Z) groups. The
measurement of total DNA of duodenal tissues which reflect the cell mitosis was
higher in (T), (Z) and (BTK), (BTZ) at P < 0.05; the recorded results of
DNA/protein of the same segment of the duodenal tissues revealed higher ratio
(higher ribosomal activity) of (T), (Z), and (BTK) groups. Cell size of the
duodenal tissues as indicated by (protein/DNA) was increased by (K) and
decrease by (T) and (Z) supplementation at P < 0.05.
Conclusion: Supplementation of
turkey rations with herbs (basil & thyme) and multienzymes (zymogen &
kemzyme) either alone or in combination produce a significant improvement in
turkeys’ performance. They may regulate feed intake, increase intestinal surface
area for more absorption and utilization of feed; and Contain different
antioxidants activity.
Author(s) Details
Sohair Y. Saleh
Department of Physiology,
Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Nahed S. El-Toukhy
Department of Physiology,
Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Hassan. I. Abass
Department of Physiology,
Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Salma I. El- Samannoudy
Department of Physiology,
Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Mohamed A. Tony
Department of Nutrition and
Clinical Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza,
Egypt.
Amin M. Hassanin
Department of Cytology and
Histology, Faculty of Veterinary Medicine, University of Sadat City, Meonofya,
Egypt.
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