Stimulation of Mutation by Gamma Irradiation in Mulberry (Morus) Genotype S54 | Chapter 12 | Advances and Trends in Agricultural Sciences Vol. 3
Abundant mulberry genotypes are accessible in nature, they are deficient in one or the other important economic trait required for silkworm Bombyx mori L. as mulberry leaf is the sole food. In the present experiment, as S54 mulberry genotype leaves are very much suited for rearing young age (Chawki) silkworms, an effort has been made to induce phytomorphological variability in S54 mulberry genotype using gamma rays. RBD Method with three replications / treatment was followed. Experiment was performed in Mulberry garden, Department of Sericulture, Jnana Bharathi Campus, Bangalore University and Mist chamber, Indian Institute of Horticultural Research (IIHR), Bangalore, Karnataka, India. Gamma ray (1kR-10Kr) was used to induce variability in juvenile twigs of mulberry for various agro-botanical characters viz., sprouting, rooting, internodal distance, leaf area, plant height etc. and leaves were exposed to biochemical analysis. Mulberry genotype S54 showed linear decrease in growth parameters with the increased gamma ray dosage and plants exhibited variability with increased rooting (81.33%), plant height (147.86 cm) and leaf area (146.22 cm2) when compared to control in M1 generation at 7kR. Mutants displaying encouraging characters were grown for M2 generation which exhibited marked enhancement in growth and yield parameters. Biochemical constituents in S54 mutant leaves recorded at 7kR revealed increased proteins, carbohydrate, chlorophyll a and b. Mulberry cuttings irradiated with gamma ray (7kR) exhibited favorable characters in rooting, plant height and leaf area over the control in M1 generation and mutants were grown for M2 generation and marked improvement in growth, yield and bio-chemical parameters were witnessed.
Dr. H. L. Ramesh
Department of Sericulture, V. V. Pura College of Science, K. R. Road, Bangalore-560004, Karnataka, India.
Dr. V. N. Yogananda Murthy
Department of Biotechnology, Azyme Biosciences Private Limited, Jayanagar, Bengaluru-560069, Karnataka, India.