Stimulation of Mutation by Gamma Irradiation in Mulberry (Morus) Genotype S54 | Chapter 12 | Advances and Trends in Agricultural Sciences Vol. 3
Abundant mulberry genotypes are
accessible in nature, they are deficient in one or the other important economic
trait required for silkworm Bombyx mori L. as mulberry leaf is the sole food.
In the present experiment, as S54 mulberry genotype leaves are very
much suited for rearing young age (Chawki) silkworms, an effort has been made
to induce phytomorphological variability in S54 mulberry genotype
using gamma rays. RBD Method with three replications / treatment was followed.
Experiment was performed in Mulberry garden, Department of Sericulture, Jnana
Bharathi Campus, Bangalore University and Mist chamber, Indian Institute of
Horticultural Research (IIHR), Bangalore, Karnataka, India. Gamma ray
(1kR-10Kr) was used to induce variability in juvenile twigs of mulberry for
various agro-botanical characters viz., sprouting, rooting, internodal
distance, leaf area, plant height etc. and leaves were exposed to biochemical
analysis. Mulberry genotype S54 showed linear decrease in growth
parameters with the increased gamma ray dosage and plants exhibited variability
with increased rooting (81.33%), plant height (147.86 cm) and leaf area (146.22
cm2) when compared to control in M1 generation at 7kR.
Mutants displaying encouraging characters were grown for M2
generation which exhibited marked enhancement in growth and yield parameters.
Biochemical constituents in S54 mutant leaves recorded at 7kR
revealed increased proteins, carbohydrate, chlorophyll a and b. Mulberry
cuttings irradiated with gamma ray (7kR) exhibited favorable characters in
rooting, plant height and leaf area over the control in M1
generation and mutants were grown for M2 generation and marked
improvement in growth, yield and bio-chemical parameters were witnessed.
Author(s) Details
Dr. H. L. Ramesh
Department of Sericulture,
V. V. Pura College of Science, K. R. Road, Bangalore-560004, Karnataka, India.
Dr. V. N. Yogananda Murthy
Department of Biotechnology,
Azyme Biosciences Private Limited, Jayanagar, Bengaluru-560069, Karnataka,
India.
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