Novel Simultaneous Separation and Quantitative Determination of Four Sartans in Presence of Hydrochlorothiazide by Isocratic RP-HPLC | Chapter 05 | Modern Advances in Pharmaceutical Research Vol. 3
A
simple, reliable, sensitive,
reproducible and novel isocratic RP-HPLC method was developed and validated for
the separation and quantitative estimation of four angiotensin II-receptor
antagonists namely Telmisartan (TELM), Losartan (LOSA), Olmesartan (OLME) and
Valsartan (VALS) that are utilized in treating hypertension alone or along with
some other drugs like thiazide diuretics mostly Hydrochlorothiazide (HCTZ). The
key important benefit of developed method was that the five individual drugs
can be determined on a single chromatographic system without alteration in
detection wavelength and mobile phase. All the above said drugs were separated
by using Welchrom C18 column having internal diameter of 4.6 X 250 mm with 5 µm
particle size as stationary phase with mobile phase consisting a mixture of acetonitrile
and phosphate buffer (pH-3.3, 50:50 v/v). The mobile phase was pumped at a flow
rate of 1 ml/min. The wave length of the UV detection was done at 238 nm. The total run time was eight minutes
and the elution window of only three minutes. The calibration curves were
linear (r2 = 0.9998) in all cases. The relative standard deviation
was less than 2% and average recovery was above 99.95%. This method was
statistically validated in different parameters like linearity, precision,
specificity, accuracy, robustness and ruggedness. The optimized method proved
to be accurate, specific and robust for the quality control of four
angiotensin-II receptor blockers alone or in combination with HCTZ in bulk drug
and tablet dosage forms.
Author(s) Details
Prof. Panchumarthy
Ravisankar
Department of Pharmaceutical
Analysis, Vignan Pharmacy College, Vadlamudi, Guntur District – 522 213, Andhra
Pradesh, India.
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