Skip to main content

Conformational Similarity between the Active Sites of a Sunflower Seedlings Lipase and a Porcine Pancreatic Lipase | Chapter 5 | Current Strategies in Biotechnology and Bioresource Technology Vol. 1


A true triacylglycerol lipase was detected in germinating sunflower (Helianthus annuus L.) seedlings associated to oleosomes. This enzyme that has not yet been identified was partially purified as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) (10%): Two protein bands, of 61 and 66 kDa, were isolated from the active lipase fraction. Polyclonal antibodies (PAbs) directed against each protein band were produced. Only PAbs against the 61 kDa protein band  (P-61) were able to inhibit the total lipolytic activity of our preparation. Hence, only the 61 kDa protein carries the sunflower lipase activity. The relationship between a pure porcine pancreatic lipase  and the P-61 purified sunflower lipolytic fraction was investigated, leading to the first evidence of            cross reaction between the porcine pancreatic lipase and the P-61 sunflower lipase fraction.These results are in agreement with common epitopes to the porcine pancreatic lipase and the sunflower P-61.
Author(s) Details –

Dr. Sellema Bahri
Laboratoire de Biochimie, Département de Biologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092, Tunis, Tunisia.

Amel Benammar-ElGaaied
Laboratoire de Génétique, Immunologie et Pathologies humaines, Département de Biologie, Faculté des Sciences de Tunis, Campus Universitaire, 2092, Tunis, Tunisia.

View Book:- http://bp.bookpi.org/index.php/bpi/catalog/book/151

Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

Risk Factors for Postpartum Psychiatric Disorders. A Review of the Literature | Chapter 8 | New Visions in Medicine and Medical Science Vol. 4

  Objective: The aim of this study was to explore the risk factors for the development of postpartum psychiatric disorders through international literature. Materials and Methods: Throughout many articles in PubMed, Google scholar and PsycInfo, a great amount of recent data was gathered to identify the disorders that are most common as well as their risk factors. Results: After childbirth, most commonly women experience postpartum depression, anxiety disorders, post-traumatic stress disorder and postpartum psychosis. All the disorders have many similar risk-factors with the main one being preexisting psychiatric disorder and many similar symptoms too. Conclusions: Women after childbirth are at risk of experience many psychiatric disorders, such as postpartum distress, postpartum post traumatic stress disorder and even more rarely postpartum psychosis. It is important to provide comprehensive support to ensure the well-being of both the mother and the infant and this will b...
Post a Comment
Read more

Alkali Element Modification of Glucose Molecules as a Method to Dissolve Cancer Cells | Chapter 12 | New Visions in Medicine and Medical Science Vol. 4

  The present study highlights about alkali element modification of glucose molecules as a method to dissolve cancer cells. The central regulation of the mechanisms governing cell proliferation has little effect on cancer cells. Cancer cells are entirely independent of the central command and divide and proliferate on their own, making it challenging to activate their response mechanism. Precisely, this is the reason why they are at risk to the health of humans and/or any biological entities. Instead of trying to reconnect the central command of the growth control mechanism to cancer cells that are already out of the range, we present a method of using the cancer cell’s own irresponsive and uncontrolled growth mechanism to their disadvantage and destroy the cancer cells. We found that this is achievable in an atomic/molecular level study of the glucose molecule, which is the primary food source used for growth and energy generation by all cells in the body, including the cancer cel...
Post a Comment
Read more