Antibacterial Activity of Mulinum spinosum Extracts against Slime-producing Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus Isolated from Nasal Carriers: An Overview | Chapter 6 | Theory and Applications of Microbiology and Biotechnology Vol. 3
Nasal carriers of Staphylococcus aureus are important
reservoirs with risk of developing endogenous infections or transmitting infections
to susceptible individuals. Methicillin-resistant S. aureus (MRSA) are
associated with higher rates of treatment failure. Some strains of S. aureus
produce slime which is believed to make the microorganisms more resistant to
antibiotics and host defenses. The antibacterial activity of ethyl acetate:
n-hexane (EtOAc:HEX) extracts of Mulinum spinosum (5:95% EtOAc:HEX, 50:50%
EtOAc:HEX, 70:30% EtOAc:HEX and mix
20:80/30:70% EtOAc:HEX, 50:50/70:30/100:0% EtOAc:HEX) were assayed against 3
slime-producing S. aureus strains and 2 MRSA strains isolated from nasal
carriers. S.aureus ATCC 35556 slime-producing strain and MRSA ATCC 43300 strain
were used as controls. The extracts were prepared using flash chromatography.
M. spinosum 5:95% AcOEt:HEX showed antibacterial effect against all
slime-producing strains (MIC:500 µg/ml) and the highest activity against MRSA
strains (MIC:500 to 1000 µg/ml). All M.spinosum extracts assayed were active
against slime-producing S. aureus and MRSA at doses between 500 and 4000 µg/ml.
Both, slime-producing S. aureus and MRSA are highly contagious and hardly
eradicated by antibiotic therapies. So, there is an increasing need to find new
substances with the ability to inhibit these strains
Author(s) Details
Echenique Daniela
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina.
Chiaramello Alejandra
Department of Chemistry, National University of San Luis, San Luis, 5700, Argentina.
Mattana Claudia
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina
Dr. Alcaráz Lucía
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina
Laciar Analía
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina
Satorres Sara
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina.
Author(s) Details
Echenique Daniela
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina.
Chiaramello Alejandra
Department of Chemistry, National University of San Luis, San Luis, 5700, Argentina.
Mattana Claudia
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina
Dr. Alcaráz Lucía
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina
Laciar Analía
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina
Satorres Sara
Department of Biochemistry and Biological Sciences, National University of San Luis, San Luis, 5700, Argentina.
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