Antimicrobial Activity and Bioactive Compound Producing Halophilic Bacteria | Chapter 10 | Theory and Applications of Microbiology and Biotechnology Vol. 3
Halophilic
bacteria are commonly found in natural environments containing significant
concentration of NaCl that harbor a number of microbes producing novel
bioactive compounds. In this study we aimed to isolate and screen out the
halophilic bacteria and to determine their activity for production of the
bioactive compounds. Marine soil sediments were collected from the solar
saltpans of Thoothukudi District, Tamil Nadu, India. Based on colony
morphology, two species were isolated and identification was done by using morphological
and biochemical tests. The extracts of cell-free supernatant of the two
halophilic isolates were screened for bioactive compound and tested for
antimicrobial activity against human pathogenic bacteria such as Staphylococcus
aureus, Pseudomonas sp, Klebsiella sp, Vibrio sp, Escherichia coli and fungi
Aspergillus niger and Penicillium chrysogenum by the agar cup diffusion method.
The results were then compared to standard antibiotics which showed 80% of
similar activity in 50 μL/g concentration. In addition, the arbitrary unit of
two isolates was calculated against S. aureus which produced enhanced
inhibitory results. These findings show that the saline soil of Thoothukudi
represents an untapped source of bacterial biodiversity and also that most
bacterial isolates are capable of antibacterial and antifungal metabolite
production.
Author(s) Details
R. K. Deepalaxmi
Department of Microbiology, St. Mary’s College (Autonomous), Tamil Nadu, India.
View Book : - http://bp.bookpi.org/index.php/bpi/catalog/book/154
Author(s) Details
R. K. Deepalaxmi
Department of Microbiology, St. Mary’s College (Autonomous), Tamil Nadu, India.
View Book : - http://bp.bookpi.org/index.php/bpi/catalog/book/154
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