Skip to main content

Quantification of Propranolol in Rat Plasma by LCMS/MS Using Tramadol as an Internal Standard for Pharmacokinetic Studies in TAA-induced Liver Fibrotic Rats | Chapter 9 | Trends in Pharmaceutical Research and Development Vol. 1

A simple, rapid and selective liquid chromatography coupled with tandem mass spectrometry (LCMS/MS) is developed and validated for quantification of propranolol without the sample extraction step in rat plasma using tramadol as an internal standard (IS). The analytes are separated using an isocratic mobile phase which consist of methanol and 10 mm ammonium formate (70/30, v/v) on an isocratic UK-C18 (Imtakt Unison 2.0 × 50 mm, 3 μm) column and was analyzed by MS/MS in the multiple reaction monitoring (MRM) mode using the transitions of respective (M+H)+ ions, m/z 260.0→116.2 and m/z 264.2→58.2 for quantification of propranolol and IS, respectively. The standard calibration curves showed good linearity within the range of 2.0 to 800.0 ng/ml (r2 = 0.999, 1/x2 weight). The lower limit of quantification (LLOQ) was 2 ng/ml. The retention time of propranolol and IS were 1.12 and 0.939 min which means that it is the potential for the high-through put potential of the proposed method. In addition, no significant metabolic compounds were found to interfere with the analysis. Acceptable precision and accuracy were obtained for the concentrations over the standard curve range. The validated method was successfully applied for the pharmacokinetic studies after 2 mg/kg of propranolol HCl in the thioacetamide (TAA)-induced fibrotic rats

Author(s) Details

Ju-Seop Kang
Department of Pharmacology, Clinical Pharmacology Lab, College of Medicine, Hanyang University, Seoul 133-791, South Korea.

View Book: - http://bp.bookpi.org/index.php/bpi/catalog/book/156

Labels:

Comments

Post a Comment

Popular posts from this blog

Greening Regional Airports: A Vision for Carbon Neutral Infrastructure | Chapter 12 | Contemporary Perspective on Science, Technology and Research Vol. 3

 This study provides an overview of the energy demand of a regional airport, divided into individual time horizons. The electrification of aircraft systems raises the question of whether airports will be among the largest electricity consumers in our infrastructure in the future. Sustainability and especially emission reductions are significant challenges for airports that are currently being addressed. The Clean Sky 2 project GENESIS addresses the environmental sustainability of hybrid-electric 50-passenger aircraft systems in a life cycle perspective to support the development of a technology roadmap for the transition to sustainable and competitive electric aircraft systems. This article originates from the GENESIS research and describes various options for ground power supply at a regional airport. Potential solutions for airport infrastructure with a short (2030), medium (2040), and long (2050) time horizon are proposed. In addition to the environmental and conservation benefi...
Post a Comment
Read more

Study on Processing Method Influences the Effect of Cassava (Manihot esculenta) Consumption on Blood Lipid Profile in Rats | Chapter 8 | Current Research and Development in Chemistry Vol.1

Cassava is consumed in many tropical countries and is fast becoming the major raw material of many diets, especially in the bakery, brewery and pharmaceutical industries. In a study to determine the effects of various formulations of Cassava feeds on blood lipid profile in rats. Forty adult Wistar rats weighing between 120 and 150 g were divided into 4 groups of 10 rats each. The rats were acclimatized for one week; fed with different formulations of Cassava feeds adlibitum, after which the rats were allotted into groups. Group 1 was fed on Garri; group 2 on Cassava flour; group 3 on Tapioca, and the control group (group 4) on normal chow. After 4 weeks, each animal was anaesthetized by exposure to chloroform vapour and blood samples collected by cardiac puncture. Group 1 diet (Garri) significantly increased HDL-cholesterol (HDL-C) concentration and significantly decreased LDL-cholesterol (LDL-C) concentration (p<0.05) compared with the control group. In Group 2 (Cassava flour), t...
Post a Comment
Read more

Ethnopharmacological Survey among Traditional Medicine Practitioners in Abidjan (Côte d'Ivoire) for the Management of Pathologies such as Malaria, ENT Diseases, Diarrhea, Typhoid Fever and Anemia | Chapter 05 | New Horizons in Medicine and Medical Research Vol. 8

 Traditional Medicine Practitioners (TMPs) of Côte d'Ivoire are familiar with the therapeutic herbs. Medicinal plants have been shown to be useful in the treatment of common diseases such as malaria (KROA, 2004) and diabetes (N'GUESSAN et al, 2013) in several studies and scientific study. In the Abidjan District, the current policy is to integrate traditional medicine and pharmacopoeia into the national health system in order to improve population health coverage, particularly in the management of commonly encountered diseases such as malaria, ENT diseases, diarrhoea, typhoid fever, and anaemia. This research aims to increase the use of traditional medicine and pharmacopoeia in the national health system. Over the course of three months, fifty (50) PMTs in the District of Abidjan participated in this study. Plant therapists accounted for 30 of the 50 PMTs polled at the end of the study, or 60%. Traditional medicine centres use 61 types of medicinal plants from 36 famili...
Post a Comment
Read more