The Details of Revamp Studies on Morpho-Histology of the Male Reproductive System of Halys dentatus Fabricious (Hemiptera: Pentatomidae) | Chapter 12 | Current Research Trends in Biological Science Vol. 1
Aim: Revamp studies on the morphology and histology of plant
bug Halys dentatus F. (Hemiptera: Pentatomidae). Study Design: Halys dentatus
Fab. is acting as pest of many plants, multivoltine in nature, therefore their
reproductive cycle observed throughout the year. Hence, the number of
reproductive cycles increased the population of H. dentatus to cause harm to
plants, therefore there is need to study the male reproductive system of H.
dentatus as a part of fundamental studies. Place and Duration of Study:
Entomology Research Laboratory; P.G. Department of Zoology, K.T.H.M. College,
Nashik. (MS, India). Methodology: Adults
of H. dentatus (Fab.) were collected, anesthetized with anesthetic ether &
embedded in dissecting paraffin wax plate, dissected in insect saline solution
(Lum, [1]) using stereoscopic research binocular microscope. The male
reproductive system exposed and isolated, fixed in Debocqui’s Bouin’s fixative
for 18 hrs., dehydrated (acetone grades), Cleared (xylene; acetone), blocks
were prepared, sections were cut on Leica microtome, stained & micro
photographed. Results and Observation: Morphology: The
male reproductive system of H. dentatus (Fab.) constitute a pair of testis,
pair of vas deferens, seminal vesicles, accessory glands (ectodermal &
endodermal) & ejaculatory duct (Bulbus & Ductus). Histology:
Histology of testis of H. dentatus showed the six numbers of testicular
follicles with different development zones; the growth zone, the maturation
zone and the zone of differentiation. The inner layer of vas deferens and
seminal vesicle was composed of cuboidal epithelial cells. The accessory
glands are both ectodermal and endodermal in origin. The ectodermal accessory
glands are triplate, milky white in colour while mesodermal accessory glands
are convoluted bunch of fine tubular structure. Conclusion: The male
reproductive system was studied with reference to revamp morpho-histology;
during the year 2009-2011. The anatomy resemble with other pentatomid bug with
little difference in vas deference, investing sac and in the number of
testicular follicles.
Author(s) Details Jyoti H. Gangurde
P. G. Department of Zoology, Entomology Research Laboratory, K. T. H. M. College, Nashik-422002, Savitribai Phule Pune University, MS, India.
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