Skip to main content

The Role of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Recognition of Pseudomonas aeruginosa in Lung Epithelial Cells: Detailed Study | Chapter 6 | Current Research Trends in Biological Science Vol. 1

Cystic Fibrosis Transmembrane Regulator (CFTR) plays an important role in chloride and thiocyanate ion homeostasis in human epithelial surfaces. Deletion of phenylalanine at position 508 (Δ508) leads to cystic fibrosis and dysregulation of pro-inflammatory cytokines. Pseudomonas aeruginosa gains predominance, contributing over 80% of the lung bacteria in adults with CF and this strongly correlates with the decline of pulmonary function and mortality. The research aimed at understanding the role of CFTR in response to P. aeruginosa, (the most common pathogen that colonises the airways of Cystic Fibrosis patients), with the objectives of evaluating the relative expression of epithelial and inflammatory cytokines (IL-17C and IL-6) in five (Calu3, CFBE41o, CFBE41o wt, Calu3 altered and Calu3 knockout) human bronchial epithelial cell lines after two hours of infection with P. aeruginosa using Quantitative Polymerase Chain Reaction (qPCR). It was found that Calu3 and Calu3 altered, treated cell lines significantly (p=0.05) increased in the level of IL-17C and IL-6 mRNA in all the experimental repeats compare to untreated.   The other three (CFBE 41o, CFBE41o wt, and Calu3 knockout) cell lines deficient of CFTR expressed low levels of these cytokines, but the level varied among the experiment in both treated and untreated cells suggesting that CFTR may modulate the level of cytokine production in bronchial epithelial cell lines. CFTR mutations have a direct effect on T cell function; enhance Th17 response which is one of the sources of IL-17. The IL-17C plays a central role in pulmonary host defence by orchestrating the accumulation and associated activity of neutrophils in the bronchoalveolar space. However, the massive neutrophils accumulation in the CF lung does not correlate with bacterial eradication but rather causes extensive tissue damage and inflammation disproportion to infection indicating that the function of neutrophils is dysregulated in CF. Therefore, knocking down IL-17C may minimise inflammation in CF patients.

Author(s) Details

Ekong, Mercy Okon
Department of Biological Sciences, Cross River University of Technology, Nigeria.

Tarh, Jacqueline Ebob
Department of Biological Sciences, Cross River University of Technology, Nigeria.

Iroegbu, Christian Ukwuoma
Department of Biological Sciences, Cross River University of Technology, Nigeria.

View Book: - http://bp.bookpi.org/index.php/bpi/catalog/book/157

Labels:

Comments

Post a Comment

Popular posts from this blog

A Prospective Study about Safety and Efficacy of Perioperative Lidocaine Infusion | Chapter 09 | New Horizons in Medicine and Medical Research Vol. 8

 Opioids cause clinically significant side effects such as respiratory depression, immunosuppression, muscle rigidity, negative inotropism, nausea, vomiting, hyperalgesia, urine retention, postoperative ileus, and drowsiness. Perioperative opioids are a major contributor to the United States' and other countries' opioid epidemics. Non-opioid analgesics, particularly lidocaine, are becoming more common for perioperative use as a result of this. A total of 185 adult patients were randomly assigned to one of two groups: control group I (105 patients) [fentanyl group] or group ii (80 patients) [opioid-free anaesthesia group]. Lidocaine 1.5 mg/kg bolus followed by 1.5 mg/kg/h infusion intraoperatively, and 1.5-2 mg/kg/h infusion for 2-8 hours postoperatively were given to patients in both groups at anaesthetic induction. Intraoperatively, both groups received analgesic adjuvants such as diclofenac 75 mg, paracetamol 1 gm, and mgso4 30-50 mg/kg. If the mean arterial pressure (map)
Post a Comment
Read more

A Brief Study of Middleware Technologies: Programming Applications and Management Systems | Chapter 15 | Novel Research Aspects in Mathematical and Computer Science Vol. 1

  Many platforms, services, applications, hardware, and operating systems are connected through the middleware layer. Because the middleware layer abstracts much low-level complexity and makes applications and software systems portable, it allows disparate systems to interface and function together in harmony. Middleware technologies enable software engineers to swiftly construct software systems and applications, allowing developers to focus on more important tasks. This chapter examines several types of middleware systems and discusses middleware capabilities, middleware operation, middleware's function in cloud-based systems, and the best middleware platforms to use. Middleware systems are widely utilised and can be found in practically any software system or application. Middleware programmes provide as a link between many sorts of systems and protocols. They serve as a mechanism for various systems. To successfully exchange information, it runs on a variety of operating system
Post a Comment
Read more

Patients’ Perspective of Acute Post-operative Pain Management: A Multicentre Survey of Tertiary Hospitals in Maharashtra, India | Chapter 08 | New Horizons in Medicine and Medical Research Vol. 8

 When postoperative pain is adequately controlled, patients' satisfaction and patient-related outcomes (PROs) increase. Understanding the patients' perspective is crucial since it supports in the formulation of improvement strategies. Because wrong attitudes and assumptions might block pain alleviation, patients' attitudes and beliefs are critical. As a result, a multicenter study of patients' attitudes, beliefs, experiences, and satisfaction levels with acute postoperative pain management was done in Maharashtra's tertiary hospitals. In addition, the responses were examined to evaluate if the Acute Pain Service (APS) resulted in improved patient outcomes and satisfaction. A 13-item questionnaire adapted from previous studies was used to capture patients' experiences with postoperative pain treatment. The responses of 179 patients are included in the study. The findings revealed that 91.6 percent of patients experienced postoperative pain, with 75.5 percent
Post a Comment
Read more