Skip to main content

TLP Sequenced and Studied for Its Functions with Targets Diagnostic and Therapeutic: Recent Perspective | Chapter 16 | Innovations in Medicine and Medical Research Vol.2

From the first analysis of immuneprecipitation followed by Western Blotting (WB) Corin and TLP seem to precipitate at the same height (approximately 50 KDa) and are recognized by the same antibodies. In parallel the tests of immunoprecipitation by the use of cell extracts derived from lung cancer cells A549 and NCI-H23 are improved with the aim to be able of obtaining a precipitate containing only the TLP. In fact the partial aminoacid sequence of TLP showes a high homology with the sequence of human Corin (only one aminoacid is different) and is present in lung cancer under different isoforms. It is known that human Corin is expressed mostly outside the cells and the protein extract derived from the extracellular medium and from the cells transfected with the plasmid, which overexpresses Corin, showes many more bands analyzed on SDS-PAGE that are equivalent to the bands (about 50-100 KDa) observed in the WB analyzed with anti-TLP. 

Author(s) Details

Giulio Tarro
Beaumont Bonelli Foundation for Cancer Research, Naples, Italy and Committee on Biotechnologies and VirusSphere, World Academy of Biomedical Technologies, UNESCO, Paris, France.

View Book : - http://bp.bookpi.org/index.php/bpi/catalog/book/161

Comments

Popular posts from this blog

Nutritional Analysis of Octopus hubbsorum B (Cephalopoda: Octopodidae) from the Pacific Ocean, Acapulco, Guerrero, México | Chapter 02 | Recent Advances in Biological Research Vol. 5

Introduction: Mexico is a country with nutrition-related problems, mainly regarding protein-energy deficit and low consumption of minerals and vitamins. This has contributed to Mexico ranking second on adult obesity within countries of the OECD (30% of the population). On the Mexican littoral, many cephalopods exist but only few octopods are used for human consumption. According to Mexican authorities, only four species of octopods are fished, although there are 15 of these benthonic zone organisms that can be used for many purposes.


Aim:
To establish the micro and macronutrient values of O. hubbsorum B and to evaluate its potential contribution to Mexican diet, since this organism is available all year in the Mexican Pacific Coast.


Study Design:
Transversal study.


Place and Duration of Study:
Samples of Octopus hubbsorum were bought from fishermen in Acapulco, in the Mexican Pacific Coast during Autumn 2016.


Methodology:
Five O. hubbsorum were bought from local fishermen during the secon…

The Utilization of Agro-Waste: A Nanobiotechnology Point of View | Chapter 10 | Recent Advances in Biological Research Vol. 5

Aims: To review the utilization of agro-waste in the eco-friendly synthesis of nanoparticles and their biomedical, catalytic and industrial applications.
Study Design: A review.
Place and Duration of Study: This review was carried out in the interim of three weeks exploiting all relevant data, literature and publications where necessary.


Methodology: Profound gathering of literatures/publications and reviews were employed with all carefulness and professional courtesy; enough useful information were gathered over time. The introduction emphasizes the dawn of science and technology, when great ideas were still latent later leading to the advent of history changing innovations like the completion of human genome and then the birth of nanotechnology. Although, nanotechnology had been known decades been popularized by Richard Feynman in his talks in the year 1959; and in this present dispensation, nanotechnology has been a solution to many intricate challenges/ threats in science and technol…

Development of Indonesian Micro, Small and Medium Enterprises and The Utilization of the Internet | Chapter 02 | Emerging Issues and Development in Economics and Trade Vol. 1

In the current era of globalization, small businesses are required to be able to compete in the market. One way for them to be able to compete is to utilize information technology in the form of Internet media. This study aims to examine recent development of micro, small, and medium enterprises (MSMEs) and to explore the use of the Internet by micro and small enterprises (MSEs) in Indonesia. For this purpose, it analyzed secondary data and primary data collected from face-to-face interviews with randomly selected 482 owners of MSEs in various cities and types of business. The secondary data analysis shows that MSMEs in Indonesia are dominated by MSEs, i.e. accounted for almost 100 per cent of all firms, but only contributed between 58 to 61 per cent of gross domestic product (GDP). Only a small fraction of MSEs in the country utilize the Internet for their businesses, although it varies by province and type of business. From the primary data analysis, it reveals three interesting fin…