Toxicological Bioassay of Petroleum Products (Kerosene) in Tri-aquatic Ecosystem Using Pollution Bio-monitor Pseudomonas sp | Chapter 1 | Theory and Applications of Microbiology and Biotechnology Vol. 3
The toxicological bioassay of petroleum products (industrial
and local ‘kpo-fire’ refined Kerosene) in tri-aquatic ecosystem (marine,
brackish and freshwater) using pollution bio-monitor Pseudomonas sp. were
investigated. The study employs experimental examination and statistical
analysis of data and interpretation. It was designed to evaluate the different
kerosene concentration and the duration of exposure that could cause potential
toxicological effect on Pseudomonas sp. in tri-aquatic ecosystem which was used
as indices to access level of pollution. Standard microbiological techniques
were used; toxicity procedure were applied using local and industrial refined
kerosene; prepared at concentrations of 1.625%, 3.25%, 6.5%, 12.5% and 25% in
fresh, brackish and marine water; total of 36 different microcosms. These were
tested with Pseudomonas sp. for 0, 4, 8, 12 and 24 h separately for each
toxicant. The cultures were incubated at 35°C for 24 hours. The median lethal
concentration (LC50) was employed to compute the toxicities of the different
toxicants on the test organism. The results specify that percentage (%)
logarithm of mortality of Pseudomonas sp. increases with increased toxicants
concentration and exposure time. The pollution bio-monitor Pseudomonas sp. demonstrated
sensitivity to the toxicity of local and industrially refined kerosene. The
sensitivity showed variations, toxic level decreased in the following order
(noting that the lower the LC50, the more toxic the toxicants): Industrial
refined kerosene in fresh water (18.79%) > Industrial refined kerosene in
brackish water (20.81%) > Local refined kerosene in brackish water (21.47%)
> Industrial refined kerosene in marine water (22.66%) > Local refined
kerosene > (24.25) > Local refined kerosene in marine water (24.94%).
Using the Pollution/Toxicity Bio-monitoring evaluation Chart; Local refined
kerosene in marine, brackish and freshwater were ‘Toxic [High], Industrial
refined kerosene in marine water was ‘Toxic [High]’ while Industrial refined
kerosene in brackish and freshwater were ‘Toxic [very High]’. Conclusion: The
study showed that industrial refined kerosene in fresh water (LC50 = 18.8%)
has the highest toxicity strength while
local refined kerosene in marine water (LC50 = 24.92%) has the least toxicity
strength on Pseudomonas sp. in the tri-aquatic ecosystem. These results show
that local and industrial refined kerosene can inhibit the growth of
Pseudomonas sp. in an aquatic
ecosystem; noting that Pseudomonas sp. is one of the most effective
biodegrading bacteria in ecological biogeochemical cycles, pollutant
removal/remediation and a key pollution bio-monitoring. Pseudomonas sp.
tolerance for hydrocarbon and its initial sensitivity per mortality within 24 hours of exposure could be accessed as
indices to measure level of pollution or toxicity of petroleum products.
Author(s) Details
Dr. Renner Renner Nrior
Department of Microbiology, Faculty of Science, Rivers State University, Port Harcourt, Nigeria.
View
Book: - http://bp.bookpi.org/index.php/bpi/catalog/book/154Author(s) Details
Dr. Renner Renner Nrior
Department of Microbiology, Faculty of Science, Rivers State University, Port Harcourt, Nigeria.
Comments
Post a Comment