Response of Pseudomonas Species from Contaminated Soils to Selected Organic (Synthetic) Pesticides | Chapter 15 | Theory and Applications of Microbiology and Biotechnology Vol. 1
Growth response profile of three
species of Pseudomonas isolated from pesticide contaminated soils within Uyo,
Nigeria was studied using standard microbiological and analytical techniques.
The ability of the isolates to tolerate varying concentrations of commercially
available pesticides namely; Decis, DD force and Cyperforce was assessed over a
96 hour period. Selective enrichment cultures with graded concentrations of the
pesticides were used to assay their growth response profile and the absorbance
determined using CO75 digital colorimeter. The results showed that the
Pseudomonas species differed biochemically. Their growth response at pesticide
concentrations 0.0001, 0.001, 0.01, 0.1, 1 and 10% v/v differed significantly
(P<0.05) at 24 hours interval for four days. At 10% concentration of Decis
pesticide over 96 hour, the absorbance were 0.20, 0.23 and 0.30 for Pseudomonas
from Agriculture research farm, hospital dumpsite and municipal waste dump site
respectively. This ability therefore offers a veritable tool for use in the
bioremediation and ultimate restoration of pesticide contaminated soils but
however requires further evaluation.
Author(s) Details
Uduak U. Ndubuisi-Nnaji
Department of Microbiology,
University of Uyo, Uyo, Nigeria.
Utibe A. Ofon
Department of Microbiology,
University of Uyo, Uyo, Nigeria.
Godwin E. Udofia
Department of Microbiology,
University of Uyo, Uyo, Nigeria.
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