Seed Germination in Wild and Cultivated Varieties of Withania somnifera (L.) Dunal | Chapter 04 | Modern Research in Botany Vol. 1
Aim:
Withania somnifera (L.) Dunal or Ashwagandha is a valuable medicinal plant
having a consistent demand in pharmaceutical industries. Traditionally it is
propagated from seeds but it’s germination capacity is poor. So in the present
study different methods were considered to sort out a suitable method that can
be used for its cultivation in the field of Hazaribag, Jharkhand, India.
Study Design: The study was done in
the laboratory and field of Hazaribag, Jharkhand, India.
Place and Duration of Study:
Department of Botany, Vinoba Bhave University, Hazaribag, Jharkhand, India. The
study was carried out from July 2012 to August 2012 and again from mid June
2013 to August 2013.
Methodology: Different pre
germination treatments including 24 hours water soaking, 48 hours water
soaking, mechanical scarification, heat treatment at 50 degree Celsius (5 min,
10 min, 15 min) and Gibberellic acid [GA3] (250 µg/l, 500 µg/l, 1000 µg/l)
treatments were proposed. The mean germination percentage and the mean
germination time were calculated for each of the treatments.
Results: The results revealed that
GA3 500 µg/l treated seeds showed increased germination percentage in
laboratory (86±0.34%) and in soil (84.1±0.36%) as well as reduced mean
germination time in laboratory (5.8±0.41 days) and in soil (10.6±0.17 days). Whereas
heat treatment at 50 degree Celsius (5 min, 10 min, 15 min) drastically reduced
germination percentage in laboratory (32±0.47%, 16±0.36%, 12±0.31%) and in soil
(51±0.49%, 49.6±0.49%, 32.2±0.46%), respectively in wild variety. In cultivated
variety the mean germination time was also more (10.36±0.18 days) in laboratory
and (13.75±0.50 days) in field as was found for wild variety. Gibberellic acid
treatment improved (83.9± 0.95%) in laboratory and (82.9±.64%) in field at 500
µg/l.
Conclusion: Pre treating fresh seeds
of Ashwagandha with GA3 500 µg/l for 24 hours before sowing can be adopted to
overcome dormancy of seeds with good germination percentage. Likewise
mechanical scarification of seeds can also be taken into consideration as an
alternative, cost-effective and eco-friendly way to break seed dormancy which
has also given favourable results.
Author(s) Details
Afshan Niyaz
Department of Botany, Vinoba
Bhave University, Hazaribag 825319, Jharkhand, India.
Enam Nabi Siddiqui
Department of Botany, Vinoba
Bhave University, Hazaribag 825319, Jharkhand, India.
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